5E41
Crystal structure of the large fragment of DNA Polymerase I from Thermus aquaticus in a closed ternary complex with 5-(N-(10-hydroxydecanoyl)-aminopentenyl)-2'-deoxyuridine-triphosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-12-12 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.000000 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 107.774, 107.774, 89.614 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.668 - 1.800 |
| R-factor | 0.1942 |
| Rwork | 0.192 |
| R-free | 0.23600 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 3m8s |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.824 |
| Data reduction software | XDS |
| Refinement software | PHENIX ((dev_2165: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 46.700 |
| High resolution limit [Å] | 1.800 |
| Number of reflections | 55908 |
| <I/σ(I)> | 13.41 |
| Completeness [%] | 99.9 |
| Redundancy | 9.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.05M Sodium cacodylate pH 6.5, 0.01M Magnesium acetate, 0.2M Ammonium acetate, 30% PEG 4000 |
