5E35
Crystal structure of H5 hemagglutinin mutant (N224K, Q226L, N158D and L133a deletion) from the influenza virus A/chicken/Vietnam/NCVD-093/2008 (H5N1) with LSTc
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-15 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.03314 |
| Spacegroup name | P 6 |
| Unit cell lengths | 130.268, 130.268, 133.590 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.633 - 2.700 |
| R-factor | 0.2321 |
| Rwork | 0.230 |
| R-free | 0.27000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5e32 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.131 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.760 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.630 | |
| Number of reflections | 34751 | |
| <I/σ(I)> | 18.5 | |
| Completeness [%] | 97.9 | 77.5 |
| Redundancy | 7.4 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 295 | 0.2 M ammonium acetate, 20% (w/v) PEG3350 |
