5E2H
Crystal Structure of D-alanine Carboxypeptidase AmpC from Mycobacterium smegmatis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97926 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 127.779, 73.682, 113.413 |
| Unit cell angles | 90.00, 90.19, 90.00 |
Refinement procedure
| Resolution | 32.551 - 1.800 |
| R-factor | 0.1651 |
| Rwork | 0.164 |
| R-free | 0.19720 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.924 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((1.10pre_2104: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.680 | |
| Number of reflections | 95577 | |
| <I/σ(I)> | 14.34 | 1.66 |
| Completeness [%] | 97.4 | 96 |
| Redundancy | 3 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.2 M Calcium Chloride, 0.1 M Tris:HCl pH 8.5, 25% (w/v) PEG 4000 |
