5E22
The second PDZ domain of Ligand of Numb protein X 2 in the presence of an electric field of ~1 MV/cm along the crystallographic x axis, with eightfold extrapolation of structure factor differences.
Experimental procedure
| Experimental method | LAUE |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 289 |
| Detector technology | CCD |
| Collection date | 2015-03-20 |
| Detector | RAYONIX MX340-HS |
| Wavelength(s) | 1.02-1.16 |
| Spacegroup name | P 1 |
| Unit cell lengths | 38.146, 38.146, 39.010 |
| Unit cell angles | 113.31, 113.31, 62.28 |
Refinement procedure
| Resolution | 28.950 - 1.797 |
| R-factor | 0.2914 |
| Rwork | 0.289 |
| R-free | 0.31270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5e11 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.803 |
| Data reduction software | Precognition |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.950 | 1.861 |
| High resolution limit [Å] | 1.797 | 1.797 |
| Rmerge | 0.076 | |
| Number of reflections | 11291 | |
| <I/σ(I)> | 6.98 | 0.67 |
| Completeness [%] | 70.2 | 17.78 |
| Redundancy | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 27-31% PEG-300, 43 mM citric acid, 35 mM NaH2PO4 |
