5E0C
Structural Insight of a Trimodular Halophilic Cellulase with a Family 46 Carbohydrate-Binding Module
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-11-19 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 120.143, 120.143, 205.327 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 65.451 - 2.351 |
| R-factor | 0.1812 |
| Rwork | 0.179 |
| R-free | 0.23150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5e09 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.985 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX (1.9_1692) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.451 | 2.435 |
| High resolution limit [Å] | 2.351 | 2.351 |
| Rmerge | 0.100 | 0.667 |
| Number of reflections | 30948 | |
| <I/σ(I)> | 19.4 | 2.5 |
| Completeness [%] | 98.2 | 95.5 |
| Redundancy | 7.7 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289.15 | 2.0 M ammonium sulfate, 5% (v/v) 2-propanol |
