5E05

Structure of Sin Nombre virus nucleoprotein in shot-axis crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-17
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9798
Spacegroup name	C 2 2 21
Unit cell lengths	49.742, 74.908, 154.359
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.438 - 2.300
R-factor	0.1849
Rwork	0.183
R-free	0.22890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5e06
RMSD bond length	0.008
RMSD bond angle	1.097
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.300
Number of reflections	13234
<I/σ(I)>	31
Completeness [%]	100.0
Redundancy	7.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	200 mM HEPES (pH 7.5), 200 mM lithium sulfate monohydrate, 23% (w/v) PEG3350