5E00
Structure of HLA-A2 P130
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-03-06 |
Detector | SDMS |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.608, 72.006, 123.788 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.826 - 1.700 |
R-factor | 0.1772 |
Rwork | 0.176 |
R-free | 0.20480 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.891 |
Data reduction software | CNS |
Data scaling software | CNS |
Phasing software | CNS |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.700 |
Number of reflections | 381837 |
<I/σ(I)> | 32.18 |
Completeness [%] | 99.4 |
Redundancy | 7.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.1mol/liter bis-Tris (pH 6.5), 20% (wt/vol) polyethylene glycol 3350 |