5DZZ

Structural characterization of intermediate filaments binding domain of desmoplakin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2006-10-11
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	111.931, 64.466, 74.044
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.620 - 2.600
R-factor	0.2115
Rwork	0.207
R-free	0.26380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lm7
RMSD bond length	0.002
RMSD bond angle	0.501
Data reduction software	SCALA
Data scaling software	SCALA (3.0.15)
Phasing software	PHASER
Refinement software	PHENIX (dev_1760)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	74.044	74.044	2.700
High resolution limit [Å]	2.600	10.730	2.600
Rmerge	0.090	0.046	0.316
Total number of observations	54886	758	1020
Number of reflections	18815
<I/σ(I)>	10.6	26.6	2.4
Completeness [%]	98.0	96.2	93
Redundancy	2.9	2.8	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	295	22% PEG MME 5000, magnesium acetate, MES