5DZV

Protocadherin alpha 7 extracellular cadherin domains 1-5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-11-06
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	1.06
Spacegroup name	I 2 2 2
Unit cell lengths	60.440, 106.450, 558.829
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.310 - 3.600
R-factor	0.2556
Rwork	0.254
R-free	0.28990
Structure solution method	SIRAS
RMSD bond length	0.003
RMSD bond angle	0.704
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	SHARP
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.310	3.690
High resolution limit [Å]	3.600	3.600
Rmerge	0.190	0.710
Number of reflections	21058
<I/σ(I)>	5.4	2.2
Completeness [%]	97.1	79.2
Redundancy	5.1	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	PEG 3350, Hepes
1	VAPOR DIFFUSION, HANGING DROP	7.5	295	PEG 3350, Hepes