5DZN

human T-cell immunoglobulin and mucin domain protein 4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-09-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	100.400, 107.297, 115.649
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.667 - 2.300
R-factor	0.2019
Rwork	0.200
R-free	0.23780
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.454
Data scaling software	HKL-2000
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.380
High resolution limit [Å]	2.300	4.950	2.300
Rmerge	0.101	0.054	0.695
Rmeas	0.110	0.059	0.758
Rpim	0.043	0.024	0.300
Total number of observations	373065
Number of reflections	57162
<I/σ(I)>	4.9
Completeness [%]	100.0	100	100
Redundancy	6.5	6.3	6.3
CC(1/2)		0.995	0.794

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	289	20% (w/v) PEG 6000, 100 mM citric acid/sodium hydroxide, pH 5.0