5DZM
HIV-1 Reverse Transcriptase RH domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-05 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 32 |
| Unit cell lengths | 56.140, 56.140, 133.749 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 48.619 - 2.050 |
| R-factor | 0.1803 |
| Rwork | 0.178 |
| R-free | 0.22480 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3k2p |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.824 |
| Data reduction software | DENZO |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.090 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.376 | |
| Number of reflections | 29211 | |
| <I/σ(I)> | 10.8 | 2.6 |
| Completeness [%] | 98.3 | 79.6 |
| Redundancy | 5.3 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M ammonium acetate, 0.1M Bis-Tris pH 5.5, 17% PEG 10,000 |
