Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-10 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 75.760, 75.760, 147.420 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.855 - 3.401 |
| R-factor | 0.2346 |
| Rwork | 0.232 |
| R-free | 0.28770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4qxw |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.908 |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.880 | 3.720 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.245 | 0.499 |
| Number of reflections | 6397 | |
| <I/σ(I)> | 6.5 | 6.5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 10.3 | 10.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 100 mM Tris 8.0, 25% PEG 3350, 12% glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
