5DZG
Crystal Structure of the catalytic nucleophile mutant of VvEG16 in complex with a xyloglucan tetradecasaccharide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-05 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.277, 78.957, 132.630 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.410 - 1.790 |
| R-factor | 0.173 |
| Rwork | 0.171 |
| R-free | 0.21700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1umz |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.479 |
| Data scaling software | XDS (January 10, 2014) |
| Phasing software | PHASER (2.5.2) |
| Refinement software | PHENIX (1.9-1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.410 | 1.830 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.097 | 0.317 |
| Number of reflections | 40942 | |
| Completeness [%] | 98.2 | 78.3 |
| Redundancy | 5.9 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 10 mg/mL Glc8 XyGOs, 1 mM EDTA, 100 mM pH 6 MES, 20% PEG 6000 |
