5DYV
AbyU - wildtype
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 193 |
| Detector technology | PIXEL |
| Collection date | 2015-05-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9730 |
| Spacegroup name | P 1 |
| Unit cell lengths | 61.800, 73.590, 86.190 |
| Unit cell angles | 112.87, 90.04, 89.98 |
Refinement procedure
| Resolution | 79.410 - 2.500 |
| R-factor | 0.19946 |
| Rwork | 0.197 |
| R-free | 0.25298 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.848 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.410 | |
| High resolution limit [Å] | 2.360 | 2.360 |
| Rmerge | 0.120 | 0.358 |
| Number of reflections | 52922 | |
| <I/σ(I)> | 6.4 | |
| Completeness [%] | 92.4 | |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.02 M magnesium chloride, 0.1 M HEPES pH 7.5 18% w/v poly(acrylic acid) sodium salt MW 5100 |
