5DYR
Structure of virulence-associated protein D (VapD) from Xylella fastidiosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-11 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.4586 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 107.930, 107.930, 53.281 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 18.690 - 3.000 |
| R-factor | 0.20183 |
| Rwork | 0.199 |
| R-free | 0.25306 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.722 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 35.100 |
| High resolution limit [Å] | 2.200 |
| Number of reflections | 15925 |
| <I/σ(I)> | 6.7 |
| Completeness [%] | 85.8 |
| Redundancy | 11.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 289 | 20% MPD, 0.1M NaAc pH 4.5, 170mM glycine |
