5DY0
Crystal of AmtR from Corynebacterium glutamicum in complex with DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-09-22 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 2 2 21 |
Unit cell lengths | 101.731, 137.423, 239.874 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 137.420 - 3.000 |
R-factor | 0.20677 |
Rwork | 0.206 |
R-free | 0.22398 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5dy1 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.385 |
Data reduction software | XDS |
Data scaling software | Aimless (0.1.29) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 119.940 | 119.940 | 3.070 |
High resolution limit [Å] | 3.000 | 14.070 | 3.000 |
Rmerge | 0.055 | 0.028 | 0.683 |
Rpim | 0.029 | 0.016 | 0.358 |
Total number of observations | 295546 | 2155 | 20906 |
Number of reflections | 67304 | ||
<I/σ(I)> | 17.1 | 39.2 | 2.1 |
Completeness [%] | 99.0 | 84.6 | 99.8 |
Redundancy | 4.4 | 3.4 | 4.6 |
CC(1/2) | 0.999 | 0.999 | 0.774 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 294 | 1.2 M Trisodium Citrate, 0.1 M NaHepes pH 6.8 |