5DXV
Crystal structure of Rethreaded DHFR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2015-06-14 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 44.817, 120.054, 111.187 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.550 |
| R-factor | 0.1864 |
| Rwork | 0.185 |
| R-free | 0.21433 |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.284 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 1.550 |
| Number of reflections | 40229 |
| <I/σ(I)> | 24.9 |
| Completeness [%] | 91.4 |
| Redundancy | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 1.0M LiCl2, 0.1M Sodium acetate, and 30% PEG 6000 |
