5DX3
Estrogen Receptor Alpha Ligand Binding Domain Y537S Mutant in Complex with Stapled Peptide SRC2-P3 and Estradiol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-08-11 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.162, 85.851, 58.764 |
| Unit cell angles | 90.00, 109.09, 90.00 |
Refinement procedure
| Resolution | 26.536 - 2.090 |
| R-factor | 0.212 |
| Rwork | 0.210 |
| R-free | 0.25710 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.537 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 26.540 |
| High resolution limit [Å] | 2.090 |
| Number of reflections | 29884 |
| <I/σ(I)> | 1.7 |
| Completeness [%] | 98.5 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 298 | 15% PEG 3,350, 200 mM MgCl2, 100 mM Tris pH 8.0 |
