5DX1

Crystal structure of CARM1, sinefungin, and PABP1 peptide (R455)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2012-08-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 21 21 2
Unit cell lengths	75.002, 98.322, 208.152
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.990 - 1.930
R-factor	0.1979
Rwork	0.196
R-free	0.23800
RMSD bond length	0.010
RMSD bond angle	1.372
Data scaling software	Aimless
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.080
High resolution limit [Å]	1.930	1.930
Rmerge	0.103	0.770
Number of reflections	110682
<I/σ(I)>	14.9	3.2
Completeness [%]	99.0	97
Redundancy	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291	0.2 M Ammonium Sulfate, 0.1 M Tris pH 8.5, 18% w/v PEG 3350