5DWY
Crystal structure of a substrate-free glutamate transporter homologue GltTk
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-04 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.00002 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 116.010, 116.010, 308.500 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.765 - 2.700 |
| R-factor | 0.1995 |
| Rwork | 0.198 |
| R-free | 0.23670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ky0 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.123 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.780 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.060 | 0.740 |
| Number of reflections | 52820 | |
| <I/σ(I)> | 11 | 2.49 |
| Completeness [%] | 79.0 | 17.9 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 40% v/v Glycerol; 20% w/v PEG 4000, 1% OG, 60mM Ca2+/Mg2+, 100mM HEPES |
