Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791829 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.733, 30.142, 60.755 |
| Unit cell angles | 90.00, 109.20, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.650 |
| R-factor | 0.224 |
| Rwork | 0.222 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4rof |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.140 | 30.140 | 1.680 |
| High resolution limit [Å] | 1.650 | 9.030 | 1.650 |
| Rmerge | 0.053 | 0.030 | 0.516 |
| Rmeas | 0.062 | 0.036 | 0.602 |
| Rpim | 0.033 | 0.020 | 0.308 |
| Total number of observations | 91039 | 511 | 4353 |
| Number of reflections | 24552 | ||
| <I/σ(I)> | 14.3 | 32.1 | 2.3 |
| Completeness [%] | 98.1 | 93 | 94.6 |
| Redundancy | 3.7 | 3 | 3.7 |
| CC(1/2) | 0.998 | 0.997 | 0.793 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 20% PEG-3350, 0.2 M sodium acetate |
