5DWK
Diacylglycerol Kinase solved by multi crystal multi orientation native SAD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-06-14 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 2.0664 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 75.290, 91.570, 143.720 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.785 - 2.601 |
R-factor | 0.2036 |
Rwork | 0.201 |
R-free | 0.24530 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.717 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELXDE |
Refinement software | PHENIX ((dev_2067: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.670 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.221 | 1.130 |
Number of reflections | 58770 | |
<I/σ(I)> | 36.46 | 4.18 |
Completeness [%] | 99.6 | 96.5 |
Redundancy | 142.9 | 55.85 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 5.6 | 277 | 3-5 %(V/V) 2-METHYL-2, 4-PENTANEDIOL, 0.1 M SODIUM CHLORIDE, 0.06 M MAGNESIUM ACETATE, 0.1 M SODIUM CITRATE/HCL PH 5.6. CRYSTALLIZED USING THE IN MESO (LIPIDIC CUBIC PHASE) METHOD AT 4 DEGREE CELSIUS WITH THE 7.8 MONOACYLGLYCEROL (7.8 MAG) AS THE HOSTING LIPID. |