5DVW

Structure of minor nucleoprotein V30 from Zaire ebolavirus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-09
Detector	RAYONIX MX-300
Wavelength(s)	0.97856
Spacegroup name	P 1
Unit cell lengths	34.090, 58.380, 66.600
Unit cell angles	96.33, 90.11, 106.98

Refinement procedure

Resolution	24.579 - 1.750
R-factor	0.1686
Rwork	0.167
R-free	0.20780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2i8b
RMSD bond length	0.010
RMSD bond angle	1.095
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	7.830	1.800
High resolution limit [Å]	1.750	5.530	1.750
Rmerge	0.073	0.043	0.457
Rmeas	0.095	0.056	0.598
Total number of observations	114699
Number of reflections	47118	943	3553
<I/σ(I)>	8.9	19.05	1.96
Completeness [%]	95.7	94.6	95.6
Redundancy	2.4		2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	290	Molecular Dimensions Morpheus screen e12: 40mM each Di-Ethyleneglycol, Tri-Ethyleneglycol, TetraEthyleneglycol, Penta-Ethyleneglycol; 12.% each MPD, PEG 1000; PEG 3350; 100mM Tris(base)/Bicine pH 8.5; EbzaA.17250.a.EW11.PD00370 at 22 mg/ml, tray 262514e12, puck otj5-3; cryo: direct