5DVO

Fc K392D/K409D homodimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	193
Detector technology	CCD
Collection date	2014-07-25
Detector	RAYONIX MX-225
Wavelength(s)	0.97931
Spacegroup name	P 43
Unit cell lengths	71.657, 71.657, 100.785
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.500
R-factor	0.1939
Rwork	0.190
R-free	0.26050
RMSD bond length	0.008
RMSD bond angle	1.247
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.20)
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0017)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	50.669	2.640
High resolution limit [Å]	2.500	7.910	2.500
Rmerge		0.045	0.772
Rmeas	0.172
Rpim	0.062	0.020	0.326
Total number of observations	136052	4248	19280
Number of reflections	17696
<I/σ(I)>	10.4	27.2	2.7
Completeness [%]	100.0	99.6	100
Redundancy	7.7	7.3	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		294	100mM Sodium Acetate pH 4.5 + 2000mM Ammonium Sulfate