5DVO
Fc K392D/K409D homodimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 193 |
| Detector technology | CCD |
| Collection date | 2014-07-25 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 43 |
| Unit cell lengths | 71.657, 71.657, 100.785 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.500 |
| R-factor | 0.1939 |
| Rwork | 0.190 |
| R-free | 0.26050 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.247 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0017) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 50.669 | 2.640 |
| High resolution limit [Å] | 2.500 | 7.910 | 2.500 |
| Rmerge | 0.045 | 0.772 | |
| Rmeas | 0.172 | ||
| Rpim | 0.062 | 0.020 | 0.326 |
| Total number of observations | 136052 | 4248 | 19280 |
| Number of reflections | 17696 | ||
| <I/σ(I)> | 10.4 | 27.2 | 2.7 |
| Completeness [%] | 100.0 | 99.6 | 100 |
| Redundancy | 7.7 | 7.3 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 294 | 100mM Sodium Acetate pH 4.5 + 2000mM Ammonium Sulfate |
