5DVN
Fc K392D/K409D homodimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 193 |
| Detector technology | CCD |
| Collection date | 2014-07-25 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 107.603, 107.603, 56.973 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.500 |
| R-factor | 0.1889 |
| Rwork | 0.186 |
| R-free | 0.24430 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.175 |
| Data reduction software | XDS |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0017) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 93.187 | 2.640 |
| High resolution limit [Å] | 2.500 | 7.910 | 2.500 |
| Rmerge | 0.034 | 0.697 | |
| Rmeas | 0.105 | ||
| Rpim | 0.033 | 0.012 | 0.342 |
| Total number of observations | 128970 | 4556 | 9799 |
| Number of reflections | 13146 | ||
| <I/σ(I)> | 18.8 | 51.3 | 2.3 |
| Completeness [%] | 97.9 | 99.6 | 86.3 |
| Redundancy | 9.8 | 9.7 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 294 | 100mM Tris pH 8.5 + 25% PEG MME 5K + 200mM Lithium Sulfate |
