5DVL
Fc Design 20.8.37 A chain homodimer Y349S/T366M/K370Y/K409V
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 193 |
| Detector technology | CCD |
| Collection date | 2014-07-10 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 110.502, 110.502, 57.872 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| R-factor | 0.2017 |
| Rwork | 0.201 |
| R-free | 0.22240 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.156 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0017) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 31.899 | 31.899 | 2.000 |
| High resolution limit [Å] | 1.900 | 6.010 | 1.900 |
| Rmerge | 0.064 | 0.742 | |
| Rmeas | 0.145 | ||
| Rpim | 0.047 | 0.025 | 0.272 |
| Total number of observations | 297787 | 9290 | 42014 |
| Number of reflections | 32319 | ||
| <I/σ(I)> | 10.1 | 19.8 | 3.2 |
| Completeness [%] | 99.9 | 97.9 | 99.8 |
| Redundancy | 9.2 | 8.6 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 294 | 100mM Tris HCl pH 8.5 + 15% PEG 20K |
