5DVK

Fc Design 7.7 B chain homodimer T366V/K409I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	193
Detector technology	CCD
Collection date	2014-07-21
Detector	RAYONIX MX-225
Wavelength(s)	0.97931
Spacegroup name	P 32 2 1
Unit cell lengths	107.870, 107.870, 56.632
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.600
R-factor	0.2373
Rwork	0.235
R-free	0.28880
RMSD bond length	0.008
RMSD bond angle	1.286
Data scaling software	SCALA (3.3.20)
Phasing software	MOLREP
Refinement software	REFMAC (5.7.0017)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	48.428	2.740
High resolution limit [Å]	2.600	8.220	2.600
Rmerge		0.072	0.803
Rmeas	0.127
Rpim	0.038	0.026	0.280
Total number of observations	129162	3912	15181
Number of reflections	11789
<I/σ(I)>	13.5	28.2	2.7
Completeness [%]	98.9	96.3	93.7
Redundancy	11	9.7	9.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		294	100mM Hepes pH 7.5 + 10% Isopropanol + 20% PEG 4K