5DV5
The fatty acid-responsive FadR repressor of Vibrio alginolyticus complex with Palmitoyl-CoA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-09-23 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.780, 88.480, 61.650 |
| Unit cell angles | 90.00, 93.13, 90.00 |
Refinement procedure
| Resolution | 45.278 - 2.400 |
| R-factor | 0.1962 |
| Rwork | 0.191 |
| R-free | 0.24740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5DV1 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.677 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.560 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.069 | 0.393 |
| Number of reflections | 20475 | |
| <I/σ(I)> | 14.3 | 3.7 |
| Completeness [%] | 92.7 | 84.1 |
| Redundancy | 4.5 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 289 | 4% Tacsimate pH 5.0, 11% PEG 3350 |
