5DUR

Influenza A virus H5 hemagglutinin globular head in complex with antibody 100F4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-26
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	88.094, 101.223, 206.301
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.500 - 2.820
R-factor	0.2101
Rwork	0.208
R-free	0.25710
RMSD bond length	0.010
RMSD bond angle	1.385
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	40.500
High resolution limit [Å]	2.820
Number of reflections	45011
<I/σ(I)>	10.5
Completeness [%]	99.8
Redundancy	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	291	4.0M Potassium formate, Tris propane PH 9.0, 2% w/v PEGME 2000