5DUM
Crystal structure of influenza A virus H5 hemagglutinin globular head in complex with the Fab of antibody 65C6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-05-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 63.539, 92.981, 134.005 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.130 - 3.003 |
| R-factor | 0.218 |
| Rwork | 0.215 |
| R-free | 0.26870 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.383 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.000 |
| Number of reflections | 16304 |
| <I/σ(I)> | 13.9 |
| Completeness [%] | 99.8 |
| Redundancy | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 291 | 2% w/v Polyethylene glycol 400, 0.1M Imidazole pH7.0, 24% w/v Polyethylene glycol monomethyl ether 5000 |
