5DU7

Crystal structure of ldtMt2 at 1.79 Angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-17
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 1
Unit cell lengths	61.644, 75.534, 94.143
Unit cell angles	89.04, 89.96, 92.76

Refinement procedure

Resolution	50.000 - 1.790
R-factor	0.1716
Rwork	0.170
R-free	0.21000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3vyn
RMSD bond length	0.020
RMSD bond angle	1.991
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.730
High resolution limit [Å]	1.700	4.610	1.700
Rmerge	0.068	0.042	0.800
Rmeas	0.078	0.049
Rpim	0.041	0.025	0.544
Total number of observations	676223
Number of reflections	153919
<I/σ(I)>	7.6
Completeness [%]	97.3	98.3	95.7
Redundancy	3.7	3.7	3.5
CC(1/2)		0.989	0.577

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	20% PEG5000MME, 0.2mM Ammonium sulfate