5DU0
Crystal structure of rsFolder in the non-fluorescent off-state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.992 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 141.290, 134.800, 51.050 |
| Unit cell angles | 90.00, 105.58, 90.00 |
Refinement procedure
| Resolution | 95.770 - 2.350 |
| R-factor | 0.21075 |
| Rwork | 0.208 |
| R-free | 0.25734 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b3p |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.054 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 95.770 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Number of reflections | 37525 | |
| <I/σ(I)> | 10.1 | 2.2 |
| Completeness [%] | 98.1 | 88.56 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1 M Tris, 20% PEG3350 |
