5DTY
Crystal structure of rsEGFP2 in the non-fluorescent off-state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-09-09 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.873 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.195, 62.809, 70.553 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.910 - 1.500 |
R-factor | 0.1807 |
Rwork | 0.178 |
R-free | 0.22380 |
RMSD bond length | 0.018 |
RMSD bond angle | 1.762 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.910 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Number of reflections | 36769 | |
<I/σ(I)> | 11.9 | 1.2 |
Completeness [%] | 98.1 | 91.59 |
Redundancy | 4.6 | 4.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.1 | 293 | 0.1 M HEPES, 1.7 M ammonium sulphate |