5DTU
Crystal structure of the RNA-helicase Prp28 from Chaetomium thermophilum bound to ADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-05-12 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.910 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 161.500, 50.400, 65.800 |
Unit cell angles | 90.00, 101.40, 90.00 |
Refinement procedure
Resolution | 45.773 - 3.199 |
R-factor | 0.2756 |
Rwork | 0.274 |
R-free | 0.28780 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4nho |
RMSD bond length | 0.006 |
RMSD bond angle | 0.992 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.773 | 3.299 |
High resolution limit [Å] | 3.199 | 3.199 |
Rmerge | 0.124 | 0.494 |
Number of reflections | 8454 | |
<I/σ(I)> | 9.17 | 2.45 |
Completeness [%] | 95.1 | 85.1 |
Redundancy | 3.58 | 3.68 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 277.15 | 200 mM NaCl, 20 % (w/v) PEG 4000, 100 mM Tris pH 8.0 |