5DTO

Dengue virus full length NS5 complexed with viral Cap 0-RNA and SAH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-05-30
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	94.660, 151.394, 69.432
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.330 - 2.603
R-factor	0.1858
Rwork	0.183
R-free	0.24360
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.779
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	47.330
High resolution limit [Å]	2.603
Number of reflections	31340
<I/σ(I)>	1.35
Completeness [%]	99.7
Redundancy	5.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	291	0.2 M magnesium acetate, 0.1 M sodium cacodylate, 10-20%(w/v) PEG 8000