5DTF
context-independent anti-hypusine antibody FabHpu98.61 in complex with hypusine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-20 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.95370 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 130.120, 75.770, 120.610 |
| Unit cell angles | 90.00, 120.66, 90.00 |
Refinement procedure
| Resolution | 37.216 - 1.900 |
| R-factor | 0.2134 |
| Rwork | 0.212 |
| R-free | 0.25350 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ds8 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.843 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.200 | 2.180 |
| High resolution limit [Å] | 1.900 | 2.100 |
| Rmerge | 0.362 | |
| Number of reflections | 77821 | |
| <I/σ(I)> | 9.9 | 2.2 |
| Completeness [%] | 97.9 | 89.1 |
| Redundancy | 3.4 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.6 | 297 | 24% PEG 1000 0.1M HEPES 7.6 10mg/ml |
