5DT9
Crystal structure of a putative D-Erythronate-4-Phosphate Dehydrogenase from Vibrio cholerae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2015-07-31 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 145.775, 145.775, 62.031 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.663 |
| R-factor | 0.1953 |
| Rwork | 0.193 |
| R-free | 0.24030 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3oet |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.385 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX (phenix.phaser) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.710 |
| High resolution limit [Å] | 2.660 | 2.660 |
| Rmerge | 0.092 | 0.462 |
| Number of reflections | 18676 | |
| <I/σ(I)> | 14.39 | |
| Completeness [%] | 94.8 | 94.9 |
| Redundancy | 4.9 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 2 M ammonium sulfate, 0.1M Hepes pH 7.5, 2% PEG400, 1 mM Glutamic acid |
