5DST
Crystal structure of human PRMT8 in complex with SAH
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2011-11-13 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9922 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 199.983, 130.943, 294.377 |
| Unit cell angles | 90.00, 106.48, 90.00 |
Refinement procedure
| Resolution | 49.650 - 2.963 |
| R-factor | 0.22542 |
| Rwork | 0.224 |
| R-free | 0.25960 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.504 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.963 |
| Number of reflections | 145707 |
| <I/σ(I)> | 9.2 |
| Completeness [%] | 99.2 |
| Redundancy | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | Tris-Cl, NaCl, PEG6000 |
