5DS6
Crystal structure the Escherichia coli Cas1-Cas2 complex bound to protospacer DNA with splayed ends
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2015-07-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.11587 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 88.020, 123.006, 196.012 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.003 - 3.352 |
| R-factor | 0.2333 |
| Rwork | 0.231 |
| R-free | 0.27510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4p6i |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.744 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.003 | 3.420 |
| High resolution limit [Å] | 3.350 | 3.350 |
| Rmerge | 0.216 | 0.943 |
| Number of reflections | 31049 | |
| <I/σ(I)> | 5 | 1.3 |
| Completeness [%] | 99.6 | 97.7 |
| Redundancy | 4.1 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 100 mM sodium citrate tribasic pH 5.6, 200 mM sodium acetate and 8% PEG 8000 (w/v) |
