5DS6

Crystal structure the Escherichia coli Cas1-Cas2 complex bound to protospacer DNA with splayed ends

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	80
Detector technology	CCD
Collection date	2015-07-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.11587
Spacegroup name	P 21 21 21
Unit cell lengths	88.020, 123.006, 196.012
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.003 - 3.352
R-factor	0.2333
Rwork	0.231
R-free	0.27510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4p6i
RMSD bond length	0.003
RMSD bond angle	0.744
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.003	3.420
High resolution limit [Å]	3.350	3.350
Rmerge	0.216	0.943
Number of reflections	31049
<I/σ(I)>	5	1.3
Completeness [%]	99.6	97.7
Redundancy	4.1	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	100 mM sodium citrate tribasic pH 5.6, 200 mM sodium acetate and 8% PEG 8000 (w/v)