5DRV

Crystal structure of the G3BP2 NTF2-like domain in complex with a peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-18
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	0.87
Spacegroup name	H 3 2
Unit cell lengths	94.850, 94.850, 114.030
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	38.641 - 2.750
R-factor	0.2503
Rwork	0.245
R-free	0.29910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fcm
RMSD bond length	0.002
RMSD bond angle	0.577
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.800	2.820
High resolution limit [Å]	2.750	2.750
Rmerge	0.146	2.120
Number of reflections	5273
<I/σ(I)>	21.9	2
Completeness [%]	99.6	99.7
Redundancy	19	20.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.4	293	0.9 M tri-sodium citrate, 20 % glycerol, 0.1 M sodium cacodylate (pH 6.4)