5DRN
Context-independent anti-hypusine antibody FabHpu24 in complex with hypusine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-10-15 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.00001 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.453, 167.350, 68.326 |
| Unit cell angles | 90.00, 98.93, 90.00 |
Refinement procedure
| Resolution | 43.000 - 1.994 |
| R-factor | 0.1852 |
| Rwork | 0.183 |
| R-free | 0.22180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hbc |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.368 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.540 | |
| Number of reflections | 63449 | |
| <I/σ(I)> | 30.7 | 2 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 3.8 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 292 | 20% PEG3350 0.1M Bis-Tris Propane pH7.0 0.2M K.Na phosphate |
