5DQR

The crystal structure of Arabidopsis 7-hydroxymethyl chlorophyll a reductase (HCAR)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-04-21
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9793
Spacegroup name	P 32
Unit cell lengths	89.105, 89.105, 273.312
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.610 - 2.700
R-factor	0.204
Rwork	0.202
R-free	0.24600
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.079	0.512
Number of reflections	66528
<I/σ(I)>	11.6	2.3
Completeness [%]	99.6	99.9
Redundancy	3.2	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	289	0.2M MgCl2, 0.1M Tris-HCl pH 8.5, 25%PEG3350(w/v), 3% EG(v/v)