5DOY

Crystal structure of the Thermus thermophilus 70S ribosome in complex with antibiotic Hygromycin A, mRNA and three tRNAs in the A, P and E sites at 2.6A resolution

This is a non-PDB format compatible entry.

Replaces:  4Z3Q

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-10-11
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97900
Spacegroup name	P 21 21 21
Unit cell lengths	208.390, 444.580, 619.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	361.136 - 2.600
R-factor	0.2358
Rwork	0.233
R-free	0.28280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4y4p
RMSD bond length	0.005
RMSD bond angle	0.957
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	361.136	361.136	2.670
High resolution limit [Å]	2.600	11.630	2.600
Rmerge	0.186	0.044	1.503
Rmeas	0.205	0.048	1.666
Total number of observations	9880724
Number of reflections	1725274	19594	127428
<I/σ(I)>	7.32	22.74	1.04
Completeness [%]	99.3	95.5	99.5
Redundancy	5.727

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME