5DOF

Crystal structure of Tetrahymena p19

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	277
Detector technology	CCD
Collection date	2014-07-09
Detector	MAR CCD 165 mm
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	40.732, 128.631, 75.328
Unit cell angles	90.00, 105.05, 90.00

Refinement procedure

Resolution	39.370 - 1.700
R-factor	0.1789
Rwork	0.177
R-free	0.22010
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.117
Data scaling software	HKL-2000
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.720
High resolution limit [Å]	1.690	4.590	1.690
Rmerge	0.075	0.043	0.657
Rmeas	0.081	0.046	0.712
Rpim	0.029	0.017	0.270
Total number of observations	619166
Number of reflections	83371
<I/σ(I)>	8.9
Completeness [%]	99.1	100	82.8
Redundancy	7.4	7.3	6.7
CC(1/2)		0.998	0.835

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7	277	4.0 M sodium formate pH 7.0, 2% (v/v) PEG 400