5DO7
Crystal Structure of the Human Sterol Transporter ABCG5/ABCG8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2013-11-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 173.552, 224.804, 253.304 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 3.930 |
| R-factor | 0.24951 |
| Rwork | 0.245 |
| R-free | 0.32871 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.642 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 3.900 |
| Number of reflections | 46342 |
| <I/σ(I)> | 8.8 |
| Completeness [%] | 99.4 |
| Redundancy | 18.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | Bicelle, ammonium sulfate, PEG 400, MES, TCEP | |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | Bicelle, ammonium sulfate, PEG 400, MES, TCEP |
