5DNW
Crystal structure of KAI2-like protein from Striga (apo state 1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 95 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-09-26 |
| Detector | RIGAKU RAXIS VII |
| Wavelength(s) | 1.542 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 75.940, 75.940, 181.470 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.740 - 2.020 |
| R-factor | 0.1798 |
| Rwork | 0.177 |
| R-free | 0.22462 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vxk |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.310 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.740 | 2.070 |
| High resolution limit [Å] | 2.020 | 2.020 |
| Rmerge | 0.370 | |
| Number of reflections | 21039 | |
| <I/σ(I)> | 36.2 | 6.1 |
| Completeness [%] | 99.6 | 96.5 |
| Redundancy | 18.7 | 9.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 278 | sodium formate, Tris |
