5DNU
Crystal structure of Striga KAI2-like protein in complex with karrikin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 95 |
Detector technology | CCD |
Collection date | 2015-06-09 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.000 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 75.757, 75.757, 181.266 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.700 - 1.200 |
R-factor | 0.14089 |
Rwork | 0.140 |
R-free | 0.15539 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5dnw |
RMSD bond length | 0.011 |
RMSD bond angle | 1.692 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0124) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.700 | 1.220 |
High resolution limit [Å] | 1.200 | 1.200 |
Rmerge | 0.390 | |
Number of reflections | 96642 | |
<I/σ(I)> | 97.8 | 5.3 |
Completeness [%] | 99.9 | 99.6 |
Redundancy | 20.6 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 278 | Sodium formate, Tris |