5DNA
Crystal structure of Candida boidinii formate dehydrogenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-04-03 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 53.711, 68.514, 109.458 |
| Unit cell angles | 78.11, 89.66, 81.24 |
Refinement procedure
| Resolution | 19.895 - 1.750 |
| R-factor | 0.1453 |
| Rwork | 0.143 |
| R-free | 0.18210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2j6i |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.266 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.900 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.061 | 0.411 |
| Number of reflections | 141899 | |
| <I/σ(I)> | 15.3 | 2.9 |
| Completeness [%] | 93.2 | 90 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.1 M sodium acetate trihydrate, 0.1 M HEPES, 25% PEG 4000, pH 7.5 |
