5DN1
Crystal structure of Phosphoribosyl isomerase A from Streptomyces coelicolor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-02 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97924 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 65.208, 65.208, 104.538 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.360 - 1.953 |
| R-factor | 0.1505 |
| Rwork | 0.148 |
| R-free | 0.18710 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.980 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.980 |
| High resolution limit [Å] | 1.950 | 5.290 | 1.950 |
| Rmerge | 0.148 | 0.059 | 0.591 |
| Rmeas | 0.160 | 0.064 | 0.646 |
| Rpim | 0.060 | 0.025 | 0.252 |
| Total number of observations | 125201 | ||
| Number of reflections | 19264 | ||
| <I/σ(I)> | 6.8 | ||
| Completeness [%] | 100.0 | 99.7 | 100 |
| Redundancy | 6.5 | 6 | 5.9 |
| CC(1/2) | 0.996 | 0.854 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 297 | 0.2M Sodium Chloride, 0.1M Sodium cacodylate, 2.0M Ammonium sulfate |
