5DMJ
Structure of the extracellular domain of the CD40 in complex with 3H56-5 DAB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-27 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 199.300, 48.700, 138.800 |
| Unit cell angles | 90.00, 118.20, 90.00 |
Refinement procedure
| Resolution | 46.260 - 2.790 |
| R-factor | 0.2299 |
| Rwork | 0.229 |
| R-free | 0.25820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | CD40 ENSEMBLE FROM 1JMA 2uwi 2aw2 1ncf 1tnr 2hev 2HEY; DAB FROM 2VYR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.360 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.6) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.790 | 2.800 |
| Rmerge | 0.091 | 0.421 |
| Number of reflections | 29303 | |
| <I/σ(I)> | 14.6 | 3.4 |
| Completeness [%] | 98.6 | 98 |
| Redundancy | 3.2 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.5 | 293 | 100mM Citric Acid, pH 3.5, 20% (w/v) PEG 4000, 200 mM potassium formate |
